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HH-8 cell line

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biological source

human kidney (embryonic)

form

liquid

growth mode

Semi-adherent aggregates

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human embryo kidney

Cell Line Description

The cell line HH-8 was generated by transfecting a vector (pcDNA3) containing a truncated calcium channel cDNA into (HEK) 293 cells (ECACC catalogue no. 85120602). The vector is constituitively expressed in the HH-8 cells producing a truncated (at amino acid 1733) cardiac α 1c-a calcium channel.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS + 200 μg/ml Geneticin

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin, trypsin/EDTA, or PBS wash, 5% CO2; 37°C. Cells may take up to 7 days to attach after resuscitation and sub culture. Cells grow in large aggregates that do not grow to confluency. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and re-seed to allow re-attachment of cells.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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C Seisenberger et al.
Naunyn-Schmiedeberg's archives of pharmacology, 352(6), 662-669 (1995-12-01)
Stable cell lines are potentially excellent tools for large-scale screening of new compounds. Two carboxyterminal-deleted constructs of the two splice variants a and b of the calcium channel class C alpha 1 subunit were expressed stably in HEK 293 cells.
S Dai et al.
FEBS letters, 442(1), 70-74 (1999-01-29)
Facilitation of calcium current by depolarizing prepulses has been observed in many cells including cardiac muscle. The mechanism underlying prepulse facilitation is controversial with respect to the requirements of channel subunits and cAMP kinase. We found that coexpression of the

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