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A2172

Sigma-Aldrich

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse

clone 5C5, ascites fluid

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Synonym(s):
Anti-ACTA
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

5C5, monoclonal

contains

15 mM sodium azide

species reactivity

rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle
immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle
indirect ELISA: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
A4700SAB4200602A2547
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

clone

5C5, monoclonal

clone

AC-40, monoclonal

clone

5C5, monoclonal

clone

1A4, monoclonal

species reactivity

rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog

species reactivity

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

species reactivity

human, carp, bovine, frog, sheep, rabbit, rat, snake, guinea pig

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle, immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry (frozen sections): suitable using methanol-fixed, frozen sections, indirect ELISA: suitable, indirect immunofluorescence: 1:200 using cultured human or chicken fibroblasts, western blot: 1:500 using cultured human or chicken fibroblast extract

technique(s)

immunoblotting: suitable, immunohistochemistry: 1-2 μg/mL using formalin-fixed, paraffin embedded human tongue, indirect ELISA: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells, immunohistochemistry (frozen sections): suitable using smooth muscle cells, indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix, western blot: suitable using smooth muscle cells

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General description

Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.

Immunogen

rabbit striated muscle actin.

Application

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used
  • immunofluorescence
  • double immunostaining
  • double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.

Biochem/physiol Actions

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Physical form

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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