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A4043

Sigma-Aldrich

Azure B

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein/REAXYS Number:
3922630
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Product Name

Azure B, certified by the BSC

agency

certified by the BSC

Quality Level

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

dark green

mp

205-210 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

fluorescence

λex 648 in ethanol

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Application

Azure B has been used for the staining of chromosomes.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

Suitability

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Nuclear DNA content, base composition, and cytogenetic characterization of Christia obcordata.
Sakhanokho HG and Islam-Faridi N
J. Am. Soc. Hort. Sci., 138, 205-209 (2013)
Bain BJ, et al.
Dacie and Lewis Practical Haematology (2016)
C J Kligora et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 12(12), 1143-1147 (2000-01-05)
Distinguishing heavily pigmented melanocytes from melanophages on routine hematoxylin and eosin slides can be difficult. Melanin bleaching with potassium permanganate solution is a traditional means of removing melanin from tissues and can be used before immunohistochemical staining to remove any
F S Archibald
Applied and environmental microbiology, 58(9), 3110-3116 (1992-09-01)
The discovery in 1983 of fungal "ligninases" capable of catalyzing the peroxidation of nonphenolic aromatic lignin components has been seen as a major advance in understanding how certain basidiomycete fungi can completely degrade lignin. The ability of these lignin-type peroxidases
Anél Petzer et al.
Toxicology and applied pharmacology, 258(3), 403-409 (2011-12-27)
Methylene blue (MB) has been shown to act at multiple cellular and molecular targets and as a result possesses diverse medical applications. Among these is a high potency reversible inhibition of monoamine oxidase A (MAO-A) that may, at least in

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