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A5106

Sigma-Aldrich

Anti-ABI1 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-Abelson interactor 1, Anti-Abl-interactor protein 1, Anti-Nap1 binding protein NAP1BP, Anti-eps8 binding protein, Anti-interactor protein AblBP4, Anti-spectrin SH3 domain binding protein 1 SSH3BP1
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human, canine (predicted), mouse (predicted), rat

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 0.5-1.0 μg/mL using A431 cell lysate
western blot: 0.5-1.0 μg/mL using extract of rat brain cytosolic fraction (S1)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABI1(10006)
mouse ... Abi1(11308)
rat ... Abi1(79249)

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This Item
HPA029973HPA035404HPA035403
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

species reactivity

human, canine (predicted), mouse (predicted), rat

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

western blot: 0.5-1.0 μg/mL using A431 cell lysate, western blot: 0.5-1.0 μg/mL using extract of rat brain cytosolic fraction (S1)

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:20-1:50

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500

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General description

Abelson interactor 1 (Abi1) protein, a 65 kD-substrate of the eponymous Abelson non-receptor tyrosine kinase (c-Abl) is expressed mainly in the central nervous system. This gene is located on human chromosome 10p12.
Abi 1 is an adaptor protein that interacts with Wave2 to regulate actin polymerization.

Immunogen

synthetic peptide corresponding to amino acids 163-180 of human ABI1, conjugated to KLH. The corresponding sequence is identical in several species including mouse, rat, and dog.

Application

Anti-ABI1 antibody produced in rabbit has been used in western blot analysis and immunofluorescence.
Rabbit anti- ABI1 antibody can be used for immunoblotting assays at a concentration of 0.5-1.0μg/ml using extracts from A431 and rat brain cells.

Biochem/physiol Actions

Abelson interactor 1 (Abi1) is essential for the formation and activation of WAVE2 signaling complex. ABI1 and WAVE are essential for Rac-dependent membrane protrusion and macropinocytosis, whereas ABI1 and N-WASP regulate actin-based vesicular transport, EGF receptor endocytosis, and EGFR and transferrin receptor (TfR) cell surface distribution. ABI1 has been shown to act as dual regulator of WAVE and neural Wiskott-Aldrich syndrome protein (N-WASP) specific activities. ABI1 is an essential component of this signaling complex. It positively regulates WAVE activity and connects WAVE to Rac through assembly of the WAVE-ABI1-Nap1-PIR121 complex. Abi1 modulates actin reorganization and lamellipodia formation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Xing Judy Chen et al.
Developmental cell, 30(5), 569-584 (2014-09-10)
Ena/VASP proteins and the WAVE regulatory complex (WRC) regulate cell motility by virtue of their ability to independently promote actin polymerization. We demonstrate that Ena/VASP and the WRC control actin polymerization in a cooperative manner through the interaction of the
Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells
Steinestel K, et al.
Molecular Cancer, 13(1), 145-145 (2014)
Hriday P Bhambhvani et al.
Neuroreport, 27(3), 145-150 (2015-12-23)
Schizophrenia is a severe psychiatric disorder that is characterized by a wide array of symptoms and a complex neuropathology. A well-characterized neurobiological feature of schizophrenia is abnormal synaptic plasticity, although the mechanisms underlying this are not fully understood. Numerous studies
Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes
Innocenti M, et al.
Nature Cell Biology, 7(10), 969-976 (2005)
Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton
Chen XJ, et al.
Developmental Cell, 30(5), 569-584 (2014)

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