IgG, a monomer, is the predominant antibody circulating in blood which assist in phagocytic destruction of foreign microorganism. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 30 000) can be used in rat MPO (myeloperoxidase) ELISA. It may also use as secondary antibody in immunodetection method. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all rat immunoglobulins.

Purified rat IgG.

Anti-rat IgG (whole molecule)–alkaline phosphatase antibody can be used in ELISA and western blot assays.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Western blot was performed to analyze purified proteins over expressed in E.coli using alkaline-phosphatase-conjugated goat anti-rat IgG at a 1:1000 dilution. Western blot was developed using 5-bromo-4-chloro-3indolyl phosphate/nitro blue tetrazolium tablets (Sigma).

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 10 mM glycine, 50% glycerol and 15 mM sodium azide.

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