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A8604

Sigma-Aldrich

Anti-Annexin V antibody, Mouse monoclonal

clone AN5, purified from hybridoma cell culture

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Synonym(s):
Annexin V Antibody, Annexin V Antibody - Monoclonal Anti-Annexin V antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AN5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa total cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ANXA5(308)

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WH0000308M1A4475V9264
Gene Information

human ... ANXA5(308)

Gene Information

human ... ANXA5(308)

Gene Information

human ... ANXA7(310)
mouse ... Anxa7(11750)
rat ... Anxa7(155423)

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

mouse, canine, rat, chicken, bovine, human, rabbit

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

clone

AN5, monoclonal

clone

1F4-1A5, monoclonal

clone

203-217/6, monoclonal

clone

hVIN-1, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

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General description

Monoclonal Anti-Annexin V (mouse IgG1 isotype) is derived from the hybridoma AN5 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with purified human Annexin V. Annexin V belongs to a class of Ca2+-dependent binding proteins. the amino acid sequence of the annexin V protein consists of a core of four repeats of a highly conserved 70 amino acid residue motif and a unique N-terminal tail. Within each repeat, there is a 17 amino acid residue consensus sequence, which is postulated to form part of the Ca2+ and/or phospholipid binding site.

Immunogen

purified human annexin V.

Application

Monoclonal Anti-Annexin V antibody produced in mouse has been used in:
  • immunocytochemistry
  • indirect immunofluorescence
  • immunohistochemistry
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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H Grassme et al.
The Journal of biological chemistry, 276(23), 20589-20596 (2001-03-30)
Clustering seems to be employed by many receptors for transmembrane signaling. Here, we show that acid sphingomyelinase (ASM)-released ceramide is essential for clustering of CD95. In vitro and in vivo, extracellularly orientated ceramide, released upon CD95-triggered translocation of ASM to
Poult Intestinal Organ Culture for Propagation of Turkey Coronavirus and Assessment of Host-virus Interactions
Gomes DE, et al.
Brazilian Journal of Veterinary Pathology, 4(1), 30-35 (2017)
Domain IV of Annexin A5 Is Critical for Binding Calcium and Guarantees Its Maximum Binding to the Phosphatidylserine Membrane
Wang J, et al.
Molecules (Basel), 22(12), 2256-2256 (2017)
Expression of pro-and-anti-apoptotic antigens in the cerebellum of dogs naturally infected with canine distemper virus
Bregano LC, et al.
Brazilian Journal of Veterinary Pathology, 3(2), 16-16 (2010)
Wolfram Brune et al.
Journal of virology, 77(21), 11633-11643 (2003-10-15)
Viruses have evolved various strategies to prevent premature apoptosis of infected host cells. Some of the viral genes mediating antiapoptotic functions have been identified by their homology to cellular genes, but others are structurally unrelated to genes of known function.

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