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B3795

Sigma-Aldrich

Brain-derived neurotrophic factor human

BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
Abrineurin, BDNF
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

quality

endotoxin tested

mol wt

~27 kDa

packaging

pkg of 10 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg Endotoxin level

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
SRP3014G1777SRP6053
BDNF human Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

Sigma-Aldrich

SRP3014

BDNF human

Sigma-Aldrich

Sigma-Aldrich

SRP6053

ProBDNF human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

lyophilized

mol wt

~27 kDa

mol wt

27.0 kDa

mol wt

~30 kDa

mol wt

~52 kDa

packaging

pkg of 10 μg, pkg of 5 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

General description

BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions

Application

Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.

Biochem/physiol Actions

Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.

Physical form

lyophilized from a sterile 0.2 μm filtered solution with BSA

Analysis Note

The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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