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B3931

Sigma-Aldrich

Bisindolylmaleimide X hydrochloride

≥90%, solid

Synonym(s):

Ro 31-8425

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About This Item

Empirical Formula (Hill Notation):
C26H24N4O2 · HCl
CAS Number:
145317-11-9
Molecular Weight:
460.96
MDL number:
MFCD04972010
UNSPSC Code:
12352111
PubChem Substance ID:
329773224
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

200

assay

≥90%

form

solid

solubility

DMSO: soluble

storage temp.

−20°C

SMILES string

Cl.Cn1cc(C2=C(C(=O)NC2=O)c3c4CC(CN)CCn4c5ccccc35)c6ccccc16

InChI

1S/C26H24N4O2.ClH/c1-29-14-18(16-6-2-4-8-19(16)29)23-24(26(32)28-25(23)31)22-17-7-3-5-9-20(17)30-11-10-15(13-27)12-21(22)30;/h2-9,14-15H,10-13,27H2,1H3,(H,28,31,32);1H

InChI key

IMBOYWXMTUUYGZ-UHFFFAOYSA-N

Application

Bisindolylmaleimide X hydrochloride has been used as a protein kinase C (PKC) inhibitor:
  • in chemotaxis assays
  • to inhibit protein kinase C and to study its effects on the expression of EGR1, NAB2, ZEBRA, and Rta
  • in the culture to study its effects on the expression of the kinase-insert domain-containing receptor (KDR)-B1, protein kinase Cθ (PKCθ)-M1a or Abl-HTa and on cell yield in baculovirus (BV)-infected insect cells

Biochem/physiol Actions

Bisindolylmaleimide X hydrochloride/Ro 31-8425, a strong and selective protein kinase C (PKC) inhibitor, can reduce the superoxide burst caused by various agonists in neutrophils. It can suppress the responses induced by cell surface receptors and phorbol esters in T cells. In humans, Ro 31-8425 can prevent neutrophil PKC in vitro with an IC50 of 5nM.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Markus Ackerknecht et al.
Frontiers in immunology, 6, 297-297 (2015-06-25)
Intravital imaging has revealed that T cells change their migratory behavior during physiological activation inside lymphoid tissue. Yet, it remains less well investigated how the intrinsic migratory capacity of activated T cells is regulated by chemokine receptor levels or other
J E Merritt et al.
Cellular signalling, 9(1), 53-57 (1997-01-01)
Previous studies implicating a role for protein kinase C (PKC) in mediating stimulation of cellular responses by physiological agonists have relied on use of non-specific inhibitors or direct stimulation of PKC by phorbol esters. However, much of this evidence is
André Strauss et al.
Protein expression and purification, 56(2), 167-176 (2007-08-28)
As exemplified by three cases, we show that the addition of a small molecular weight inhibitor to the culture of Baculovirus-infected insect cells can dramatically improve the expression of a recombinant kinase. The expression of the tyrosine kinase KDR was
Jianjiang Ye et al.
Journal of virology, 84(23), 12405-12418 (2010-09-24)
The Epstein-Barr virus (EBV) lytic activator genes bzlf1 and brlf1 are conventionally referred to as immediate-early (IE) genes. However, previous studies showed that the earliest expression of these genes was blocked by cycloheximide when the EBV lytic cycle was induced

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