B3934
Biuret reagent
protein detection level 150-1,000 μg/mL
Synonym(s):
Micro Lowry, Onishi & Barr Modification
Product Name
Biuret reagent, protein detection level 150-1,000 μg/mL
form
liquid
protein detection level measuring range
150-1,000 μg/mL
storage temp.
2-8°C
Quality Level
Application
Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.
General description
Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Proteins and Albumin
Roger LB
Laboratory Medicine, 45(1), e25?e41-e25?e41 (2014)
Non-lethal determination of sex and reproductive condition of Eastern oysters Crassostrea virginica Gmelin using protein profiles of hemolymph by Proteinchip textregistered and SELDI-TOF-MS technology
Li Y, et al.
Aquaculture (Amsterdam, Netherlands), 309(1-4), 258-264 (2010)
Bacterial inclusion bodies as potential synthetic devices for pathogen recognition and a therapeutic substance release
Talafova K, et al.
Microbial cell factories, 12(1), 16-16 (2013)
A simplified method of quantitating protein using the biuret and phenol reagents.
S T Ohnishi et al.
Analytical biochemistry, 86(1), 193-200 (1978-05-01)
Production of volatile fatty acids by fermentation of waste activated sludge pre-treated in full-scale thermal hydrolysis plants
Morgan-Sagastume F, et al.
Bioresource Technology, 102(3), 3089-3097 (2011)
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