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B7906

Sigma-Aldrich

1-Butanol

for molecular biology, ≥99%

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Synonym(s):
n-Butanol, Butyl alcohol
Linear Formula:
CH3(CH2)3OH
CAS Number:
Molecular Weight:
74.12
Beilstein:
969148
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

vapor density

2.55 (vs air)

vapor pressure

5 (4 mmHg) at 20 °C hPa

Assay

≥99%

form

liquid

autoignition temp.

649 °F

expl. lim.

11.2 %

color

colorless

refractive index

n20/D 1.399 (lit.)

pH

7 (20 °C, 70 g/L)

bp

116-118 °C (lit.)

mp

−90 °C (lit.)

density

0.81 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

CCCCO

InChI

1S/C4H10O/c1-2-3-4-5/h5H,2-4H2,1H3

InChI key

LRHPLDYGYMQRHN-UHFFFAOYSA-N

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This Item
901351PHR1209RTC000092
1-Butanol for molecular biology, ≥99%

Sigma-Aldrich

B7906

1-Butanol

1-Butanol BioRenewable, ACS reagent, ≥99.4%

Sigma-Aldrich

901351

1-Butanol

1-Butanol Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1209

1-Butanol

1-Butanol Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

RTC000092

1-Butanol

vapor density

2.55 (vs air)

vapor density

2.55 (vs air)

vapor density

2.55 (vs air)

vapor density

2.55 (vs air)

vapor pressure

5 (4 mmHg) at 20 °C hPa

vapor pressure

-

vapor pressure

-

vapor pressure

-

assay

≥99%

assay

≥99.4%

assay

-

assay

-

form

liquid

form

liquid

form

-

form

-

autoignition temp.

649 °F

autoignition temp.

649 °F

autoignition temp.

649 °F

autoignition temp.

649 °F

General description

1-Butanol is a clear, colorless alcohol that can be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation. It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions, thus improving recovery by ethanol precipitation.

Application

1-Butanol has been used:
  • in the derivatization process for measuring methylated arginine levels in blood samples
  • in liquid-chromatography mass spectrometry (LC-MS) analysis of lysine modifications by deacetylase CobB
  • in the extraction of plant material from Vigna unguiculata
  • to extract and purify amplified human DNA samples before further amplification by PCR followed by sequencing

1-Butanol is suitable for use in nucleic acid purification and concentration protocols.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

95.0 °F - Pensky-Martens closed cup

Flash Point(C)

35 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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