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C0897

Sigma-Aldrich

Citrate Lyase from Klebsiella pneumoniae

lyophilized powder, ≥0.20 unit/mg solid

Synonym(s):

Citrate oxaloacetate lyase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥0.20 unit/mg solid

mol wt

>5 kDa

storage temp.

2-8°C

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Application

Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).

Biochem/physiol Actions

Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).

Unit Definition

One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
Celia W Goulding et al.
Journal of molecular biology, 365(2), 275-283 (2006-10-27)
Fatty acid biosynthesis is essential for the survival of Mycobacterium tuberculosis and acetyl-coenzyme A (acetyl-CoA) is an essential precursor in this pathway. We have determined the 3-D crystal structure of M. tuberculosis citrate lyase beta-subunit (CitE), which as annotated should
M Isabel Burguete et al.
Dalton transactions (Cambridge, England : 2003), (36)(36), 4027-4033 (2007-09-11)
A turn-on fluorescent indicator for citric acid (citrate) has been developed, displaying high emission enhancement (+1500%) and low interference by other carboxylates. The sensor is based on the non-emissive copper(II) complex of a fluorescent amino amide, which, upon addition of

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