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C-12003

Sigma-Aldrich

Normal Human Epidermal Keratinocytes (NHEK)

Adult, single donor, 500,000 cryopreserved cells

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Synonym(s):
NHEK cells

biological source

human epidermis

packaging

pkg of 500,000 cells

morphology

( epidermal)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12001C-12006C-14001
packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

morphology

( epidermal)

morphology

( epidermal)

morphology

( epidermal)

morphology

(epidermal)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Skin

Application

Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of adult skin (different locations) from a single donor and are provided in a cryopreserved format. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Quality

Rigid quality control tests are performed for each lot of Normal Human Epidermal Keratinocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, immunohistochemical tests for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 5000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Primary Cell Culture Basics

What are primary cells? A comprehensive overview of animal and human primary cell lines and media and how they are used in biomedical cell culture applications. Primary cultures are less homogeneous relative to cell lines.

Primary Human Epidermal Keratinocytes Cell Culture System

Primary human keratinocytes and serum-free keratinocyte growth media for the feeder cell-free in vitro cultivation of epidermal keratinocytes. Protocols for handling, cell passaging, media, and product use.

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