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C-12915

Sigma-Aldrich

Human M2 Macrophages (hMDM-MCSF(-))

Monocyte-derived, single donor, 1.5 million cryopreserved cells

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Synonym(s):
hMDM-MCSF(-) cells

biological source

human blood (monocyte)

packaging

pkg of 1,500,000 cells

morphology

(macrophages)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12917C-12916C-12907
packaging

pkg of 1,500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 25,000,000 cells

morphology

(macrophages)

morphology

(macrophages)

morphology

(macrophages)

morphology

(mononuclear)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Monocyte

Application

Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 1.5 million viable cells after thawing.

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 200000 cells per cm2Tested Markers: CD163 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

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