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Monoclonal Anti-Calcineurin (α-Subunit) antibody produced in mouse

clone CN-A1, ascites fluid

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MDL number:
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CN-A1, monoclonal

contains

15 mM sodium azide

species reactivity

human, bovine, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human ganglia
indirect ELISA: suitable
microarray: suitable
western blot: 1:10,000 using rat brain extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PPP3R1(5534)
rat ... Ppp3r1(29748)

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C0581D219C8093
Gene Information

human ... PPP3R1(5534)
rat ... Ppp3r1(29748)

Gene Information

human ... PPP3R1(5534), PPP3R2(5535)
rat ... Ppp3r1(29748), Ppp3r2(29749)

Gene Information

mouse ... Cacna2d1(12293)
rat ... Cacna2d1(25399)

Gene Information

human ... GJA1(2697), GJA1P1(2698)
mouse ... Gja1(14609)
rat ... Gja1(24392)

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

clone

CN-A1, monoclonal

clone

CN-B1, monoclonal

clone

20A, monoclonal

clone

CXN-6, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

Calcineurin (CaN)(heterodimeric enzyme) is a Ca2+/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca2+-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, α (PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit calcineurin B is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.
Monoclonal Anti-Calcineurin (α-subunit) (mouse IgG1 isotype) is derived from the CN-A1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

Specificity

In immunoblotting, the product recognizes an epitope located on the α-subunit of calcineurin (61 kDa, also called calcineurin A) and does not cross-react with the β-subunit.

Immunogen

bovine brain calcineurin

Application

Monoclonal Anti-Calcineurin (α-Subunit) antibody produced in mouse has been used in:enzyme linked immuno sorbent assay (ELISA) ,immunohistochemistry, western blotting

Biochem/physiol Actions

Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Colin Rickman et al.
The Journal of biological chemistry, 279(13), 12574-12579 (2004-01-08)
Synaptotagmins are membrane proteins that possess tandem C2 domains and play an important role in regulated membrane fusion in metazoan organisms. Here we show that both synaptotagmins I and II, the two major neuronal isoforms, can interact with the syntaxin/synaptosomal-associated
H Bito et al.
Cell, 87(7), 1203-1214 (1996-12-27)
While changes in gene expression are critical for many brain functions, including long-term memory, little is known about the cellular processes that mediate stimulus-transcription coupling at central synapses. In studying the signaling pathways by which synaptic inputs control the phosphorylation
Yasuko Hirakawa et al.
Journal of molecular signaling, 4, 6-6 (2009-09-04)
Glucocorticoid hormones (GCs) induce apoptosis of leukemic T-cells by transcriptional regulation via the GC receptor, GR. In the human leukemic CEM cell culture model, RCAN1 has been identified as one of the genes that is specifically upregulated only in the
Anindit Mukherjee et al.
American journal of physiology. Renal physiology, 320(5), F719-F733 (2021-03-16)
Phosphorylation of the thiazide-sensitive NaCl cotransporter (NCC) in the distal convoluted tubule (DCT) is altered rapidly in response to changes in extracellular K+ concentration ([K+]). High extracellular [K+] is believed to activate specific phosphatases to dephosphorylate NCC, thereby reducing its
Impaired CuZn Superoxide Dismutase (SOD1) and Calcineurin (Cn) Interaction in ALS: A Presumed Consequence for TDP-43 and Zinc Aggregation in Tg SOD1G93A Rodent Spinal Cord Tissue
Kim Jm, et al.
Neurochemical Research, 44(1), 228-233 (2019)

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