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C213150

Sigma-Aldrich

DMT-2′O-Methyl-rC(ac) Phosphoramidite

configured for ABI

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Synonym(s):
N-acetyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-cytidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
Empirical Formula (Hill Notation):
C42H52N5O9P
CAS Number:
Molecular Weight:
801.86
NACRES:
NA.51

biological source

non-animal source (no BSE/TSE risk)

Quality Level

product line

Proligo Reagents

assay

≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)

form

powder

impurities

≤0.3% mC2 (reversed phase HPLC, Hydrolysate)
≤0.3% mC3 (reversed phase HPLC, DMT-rC(ac)me)
≤0.3% mC4 (reversed phase HPLC, DMT-rC(ac)me-Ac)
≤0.3% water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (redox titration)
≤1.0% mC1 (redox titration, DMT-rC(ac)me-DMT)
≤3% residual Solvent content

color

white to off-white

suitability

conforms to structure for H-NMR
conforms to structure for LC-MS

compatibility

configured for ABI

storage temp.

2-8°C

InChI

1S/C42H52N5O9P/c1-28(2)47(29(3)4)57(54-26-12-24-43)56-38-36(55-40(39(38)52-8)46-25-23-37(44-30(5)48)45-41(46)49)27-53-42(31-13-10-9-11-14-31,32-15-19-34(50-6)20-16-32)33-17-21-35(51-7)22-18-33/h9-11,13-23,25,28-29,36,38-40H,12,26-27H2,1-8H3,(H,44,45,48,49)/t36-,38-,39?,40-,57?/m1/s1

InChI key

WNWUMIPFLOKTEZ-IXZONTHDSA-N

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General description

DMT-2′O-Methyl-rC(ac) Phosphoramidite is a 2′O-Methyl RNA monomer that is compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. Modern phosphoramidite mediated synthesis has enabled routine high-yielding preparations of 2′O-Methyl RNA oligonucleotides. High-quality RNA phosphoramidites are key to low failure rates, the high biological activity of synthesis products, and cost-effectiveness. 2′O-Methyl RNA is a nucleic acid analog that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids.

Application

2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rC(ac) Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:
  • Diagnostic probes
  • Aptamer and ribozyme development
  • Mixed 2′O-Methyl-RNA/DNA antisense molecules

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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