MilliporeSigma
All Photos(1)

Documents

C-39220

Sigma-Aldrich

Endothelial Cell Growth Medium MV

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

Sign Into View Organizational & Contract Pricing

Synonym(s):
Recommended with C-22220

form

liquid

packaging

pkg of 1 kit

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C-39225C-22120C-39221
packaging

pkg of 1 kit

packaging

pkg of 1 vial

packaging

pkg of 500 ml

packaging

pkg of 1 kit

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

General description

Endothelial Cell Growth Medium MV SupplementPack (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-22220

Application

Endothelial Cell Growth Medium MV was developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.Recommended for:Human Dermal Microvascular Endothelial Cells (HDMEC)Human Coronary Artery Endothelial Cells (HCAEC)Human Aortic Endothelial Cells (HAoEC)Human Pulmonary Microvascular Endothelial Cells (HPMEC)Human Dermal Lymphatic Endothelial Cells (HDLEC)Human Dermal Blood Endothelial Cells (HDBEC)Human Cardiac Microvascular Endothelial Cells (HCMEC)Human Uterine Microvascular Endothelial Cells (HUtMEC)Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Endothelial Cell Media MV/MV2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEndothelial Cell Growth Supplement: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 10 ng / mlHeparin: 90 µg / mlHydrocortisone: 1 µg / ml

Storage and Stability

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.Note: The SupplementPack is delivered thawed and can be frozen after arrival without losing any activity.

Subculture Routine

Click here for more information.

recommended

Product No.
Description
Pricing

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Cuicui Guo et al.
Journal of separation science, 44(16), 3052-3060 (2021-06-09)
Human exposure to bisphenols has rarely been reported. The most important challenges in this regard are the sensitivity and accuracy of the analytical methods employed. Dansyl chloride derivatization prior to high-performance liquid chromatography-tandem mass spectrometry has been prevalently employed to
M Reza Anari et al.
Analytical chemistry, 74(16), 4136-4144 (2002-08-30)
The extensive metabolism and administration of low doses of ethinylestradiol (EE) in preclinical animal species necessitates a sensitive analytical method to quantify the drug at low picogram-per-milliliter concentrations in biological matrixes. A highly sensitive and accurate method based on the
Bianca Patrascu et al.
International journal of molecular sciences, 21(10) (2020-05-24)
Starting from dansyl-chloride, in reaction with 1,1-diphenylhydrazine and methoxyamine, two new fluorescent derivatives 1 and 2 were obtained and characterized by NMR, IR, UV-Vis, HR-MS, and fluorescence spectroscopy. The single-crystal X-ray structure was obtained for compound 2. Both compounds generate

Protocols

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Related Content

Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service