C-39620
Follicle Dermal Papilla Cell Growth Medium
SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml
Synonym(s):
Recommended with C-26500
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About This Item
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form
liquid
packaging
pkg of 1 kit
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Follicle Dermal Papilla Cell Growth Medium SupplementPack (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-26500
To form a complete medium, it should be combined with the basal medium: C-26500
Application
PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of Human Follicle Dermal Papilla Cells. The medium is optimized for primary cultures of fetal, neonatal, and adult tissue, or for the maintenance of follicle dermal papilla cells.Recommended for:Human Follicle Dermal Papilla Cells
Components
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Quality
All lots of PromoCell Follicle Dermal Papilla Cell Growth Medium are subjected to comprehensive quality control tests using primary human follicledermal papilla cells. Each lot is checked for growth promoting activity, adherence rate and typical morphology of the tested follicle dermal papilla cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Preparation Note
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.04 ml / mlBovine Pituitary Extract: 0.004 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml
Storage and Stability
Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Subculture Routine
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Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.
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