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C-43080

Sigma-Aldrich

NCCD Reagent

2 ml

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form

aqueous solution

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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This Item
GERPN30041.09028SBPVR4
NCCD Reagent 2 ml

Sigma-Aldrich

C-43080

NCCD Reagent

ECL™ Detection Reagents Cytiva RPN3004, pack of 1 ea

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Supelco

Supelco

1.09028

Nessler′s reagent

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

photometry: suitable

technique(s)

-

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

dry ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

storage temp.

−70°C

General description

NCCD Reagent

Application

NCCD Reagent is intended exclusively for use in combination with the PromoCell Primary Cancer Cell Culture Medium D-ACF (C-28081). It allows for the culture vessel surface pre-treatment which is critical for the functionality of the Primary Cancer Cell Culture System.

Quality

All lots of PromoCell NCCD Reagent are subjected to comprehensive quality control tests. Each lot is routinely tested for functionality using a human cancer cell line in conjunction with the Primary Cancer Culture Medium D-ACF. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

The NCCD Reagent is provided as a 20x stock and must be diluted to a working concentration before use. One 2 ml vial of NCCD Reagent yields 40 ml of ready-to-use working solution sufficient to treat 400 cm2 of culture surface, e.g. seven complete 6-well plates.Dilute the thawed NCCD Reagent stock solution 1:20 with PBS w/o Ca2+/Mg2+. Use 100 uL per cm2 of culture surface to treat the tissue culture vessel with the diluted NCCD Reagent and leave the closed vessel for at least 1 hour at RT. Make sure that the NCCD covers the entire vessel surface. Aspirate the NCCD solution just before seeding the cells.

Storage and Stability

Store at -20°C immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label. Unless used immediately, the sealed vessel containing the NCCD Reagent may be stored for up to 3 months at 2 to 8°C for later use. Diluted NCCD Reagent solution may be stored for up to 4 weeks at 2 to 8°C. Protect from light.

Subculture Routine

Click here for more information.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg


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Related Content

The PromoCell Primary Cancer Culture System allows selective isolation of malignant cancer cells from tumor associated stromal cells in patient derived xenographs (PDX models) and tumor samples.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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