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C9210

Sigma-Aldrich

Cyanogen bromide-activated Agarose

lyophilized powder

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Synonym(s):
Agarose, cyclic carbonimidate, CNBr-Activated Agarose
CAS Number:
68987-32-6
MDL number:
MFCD00163385
NACRES:
NA.56

form

lyophilized powder

Quality Level

200

matrix

cross-linked 4% beaded agarose

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 2.5-4.5 mL

capacity

≥10 mg/mL binding capacity (BSA)(via amino groups)

storage temp.

−20°C

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1 of 4

This Item
A6112V32541.01236
Cyanogen bromide-activated Agarose lyophilized powder

Sigma-Aldrich

C9210

Cyanogen bromide-activated Agarose

Sigma-Aldrich

Sigma-Aldrich

A6112

Calmodulin-Agarose

Supelco

Supelco

V3254

Vitamin B12-Agarose

Millipore

Millipore

1.01236

Agarose

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C

matrix

cross-linked 4% beaded agarose

matrix

4% beaded agarose

matrix

4% beaded agarose

matrix

-

matrix active group

cyanate or related structures

matrix active group

-

matrix active group

-

matrix active group

-

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

matrix spacer

1 atom

matrix spacer

8 atoms

matrix spacer

-

swelling

1 g swells to 2.5-4.5 mL

swelling

-

swelling

-

swelling

-

Application

Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
N A Morjana et al.
Protein expression and purification, 5(2), 144-148 (1994-04-01)
Protein disulfide isomerase (PDI) catalyzes the formation and rearrangement of disulfide bonds during protein folding. PDI coupled to cyanogen bromide-activated agarose retains its catalytic activity, and a column of this material increases both the rate and the yield for folding
Martiniano M Ricardi et al.
BMC plant biology, 14, 29-29 (2014-01-16)
Identifying the target genes of transcription factors is important for unraveling regulatory networks in all types of organisms. Our interest was precisely to uncover the spectrum of loci regulated by a widespread plant transcription factor involved in physiological adaptation to
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
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