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CAS9NEO

Sigma-Aldrich

Cas9 Neomycin Lenti Plasmid

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NACRES:
NA.51

recombinant

expressed in E. coli

Quality Level

form

liquid

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

selection

blasticidin

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

This product is a neomycin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link above to order gRNA.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Features and Benefits

Use lentiviral particles to generate cells with stable expression of Cas9 nuclease.

Components

1ug of plasmid DNA, EF1a-Cas9-2A-Neomycin Lenti

Linkage

Click here to order gRNA plasmid and virus.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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