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product name
MPK, 87032604
biological source
mini pig kidney
growth mode
Adherent
karyotype
2n = 38, diploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Minipig kidney
Cell Line Description
Established from the kidney of a 118 day old foetal minipig. The cells are susceptible to some arboviruses and leptospiras and highly refractory to Chlamydia spp. and meningopneumonitis.
Application
Virus studies: arboviruses
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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