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About This Item
UNSPSC Code:
41106514
Biological source:
Rat x Rat Hybridoma
Isotype:
IgG1
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Y3Ag1.2.3
Reactivity:
Anti-p21 Protein, Harvey Murine Sarcoma Virus
biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 89111412-DNA-5UG, pkg of vial of cells 89111412-1VL
ImmunoDon
Osborne-Mendel rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-p21 Protein, Harvey Murine Sarcoma Virus
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Ha-MuSV transformed syngeneic NRK cells
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen
The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask
Other Notes
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