U-2 OS Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92022711, human bone, Not specified

• Synonym(s): U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells
• UNSPSC Code: 41106514

Properties

• biological source: human bone
• packaging: tube of 5 μg 92022711-DNA-5UG; pkg of vial of cells 92022711-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

U-2 OS has been used to study:

• the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
• calcium-mediated actin reset (CaAR) in response to physiological changes

Biochem/physiol Actions

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Human Osteosarcoma

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Preparation Note

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Other Notes

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