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CLL1158

Sigma-Aldrich

A549 Cells GFP-STAT1

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NACRES:
NA.81

biological source

human male lung (Source Disease: Lung carcinoma)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... STAT1(6772)

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A549 Cells GFP-STAT1

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storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... STAT1(6772)

Gene Information

human ... SMAD4(4089)

Gene Information

human ... CTNNB1(1499), LMNB1(4001)

Gene Information

human ... STAT3(6774)

OMIM accession no.

600555

OMIM accession no.

OMIM accession no.

116806, 150340

OMIM accession no.

102582

General description

A549 GFP-STAT1 are lung carcinoma cells from a human 58 year old caucasian male having two distinct ZFN modifications creating two fluorescent transgenes each expressed from their endogenous gene locus.

This cell line was derived from ATCC Catalog No. CCL-185.

Application

This product is a human A549 cell line in which the genomic STAT1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® zinc finger nuclease (ZFN) technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the C-terminus of STAT1. Fluorescence imaging shows STAT1-GFP expression throughout the cell. Upon activation with a ligand such as interferon-gamma (INF-γ), the cell line shows redistribution of STAT1 from the cytoplasm to the nucleus, making it useful for high content screening of compounds that modulate STAT1 activity. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage

Features and Benefits

Zinc Finger Nuclease (ZFN)-mediated targeted integration of a fluorescent GFP tag into the last coding exon just preceding the stop codon of the STAT1 gene on chromosome 2q32.2 to create a cell line exhibiting stable expression of the GFP transgene.

The A549 cells are adherent, with a doubling time of approx. 22 hours.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Preparation Note

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:8

The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2 mM.

Cell freezing medium-DMSO 1X, Cat. No. C6164.

Legal Information

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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