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CRISPR20V

Sigma-Aldrich

Lenti CRISPR Universal Non-Target Control#3 Transduction Particles (LV04 vector)

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NACRES:
NA.51

form

liquid

Quality Level

packaging

vial of 200 μL

concentration

(1x106 TU/mL (via p24 titering assay))

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

General description

The Lenti CRISPR Non-Target Control#3 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#3 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#3 uses a dual-component system consisting of U6-driven guide RNA (non-target#3) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Application

Functional Genomics/Screening/Target Validation

Other Notes

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. Now complete knockout is achievable with two optimized gRNAs per gene. Minimized clone number ensures the most specific screening possible while controlling time and cost.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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