DUO96040
Duolink® PLA Multicolor Probemaker Kit - Far Red
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
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technique(s)
immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
fluorescence
λex 646 nm; λem 664 nm (Far Red)
λex 646 nm; λem 664 nm
suitability
suitable for fluorescence
shipped in
dry ice
storage temp.
−20°C
Application
Far Red fluorescence detection reagents are often used with Cy®5 filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- Detect multiple PPI, PTM and low abundant protein events in a single sample.
- Visualize protein complex formation and dissociation
- Monitor protein signaling pathways
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Detection of low abundant proteins due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Faster and more simple than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Components
Duolink® PLA Multicolor Probemaker Kit - Far Red contains all the necessary reagents to perform the conjugations of Far Red Oligo H and Far Red Oligo I directly to primary antibodies. The resulting PLA probes (Far Red H and Far Red I) must be used together and cannot be paired with any other Duolink® PLA probe.
This product is comprised of the following:
- Far Red Oligo H DUO86040H
- Far Red Oligo I DUO86040I
- Conjugation Buffer DUO82033
- Stop Reagent DUO82034
- Storage Solution DUO82035
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
Legal Information
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Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
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WGK 3
Certificates of Analysis (COA)
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Articles
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Protocols
Duolink® PLA Multicolor Detection Protocol
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