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E8022

Sigma-Aldrich

pRc/RSV Expression vector

set of expression vectors powered by either the CMV2 or the RSV promoter

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1 EA
$57.00

About This Item

MDL number:
UNSPSC Code:
41106617
NACRES:
NA.85

$57.00


In StockDetails


grade

for molecular biology

form

buffered aqueous solution

storage temp.

−20°C

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This Item
R6891R7016R8891
feature

Holds 40 x 25-mm tubes

feature

Holds 40 x 20-mm tubes

feature

Holds 40 x 25-mm tubes

feature

Holds 40 x 25-mm tubes

material

polyketone

material

polyketone

material

polyketone

material

polyketone

packaging

pack of 1 ea

packaging

pack of 1 ea

packaging

pack of 1 ea

packaging

pack of 1 ea

L × W × H

300 mm × 121 mm × 92 mm

L × W × H

250 mm × 102 mm × 83 mm

L × W × H

300 mm × 121 mm × 92 mm

L × W × H

300 mm × 121 mm × 92 mm

color

yellow

color

orange

color

orange

color

green

General description

pRc and pRSV Supercoiled Expression Vectors (5.2 kb) are for protein expression in mammalian cells. Each vector contains:
  • The polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
  • The SV40 replication origin for episomal replication
  • The f1 origin for rescue of the sense strand
  • The neomycin resistance gene, and
  • An ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.


The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.

The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.

The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.

Specificity

The MCS contains unique ApaI (CMV2 only), HindIII, NotI, SpeI (RSV only), and XbaI restriction sites, plus redundant BstXI sites.

Features and Benefits

Supercoiled vectors for protein expression in mammalian cells. Both of these vectors contain:
  • the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
  • the SV40 replication origin for episomal replication
  • the f1 origin for rescue of the sense strand
  • the neomycin resistance gene, and
  • an ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
The primary difference between these expression vectors is that one contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and the other from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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