Skip to Content
MilliporeSigma
All Photos(1)

Documents

EMS0007

Sigma-Aldrich

Recombinant Trypsin Dimethlyated

Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris

Synonym(s):

Dimethylated rTrypsin, Mass Spectrometry Trypsin, Proteomics grade rTrypsin

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352204
NACRES:
NA.26

recombinant

expressed in Pichia pastoris

Quality Level

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥10,000 units/mg protein

suitability

suitable for mass spectrometry

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0.

This product is prepared from recombinant trypsin, porcine sequence and the lysine residues have been dimethylated to further restrict autolysis. It is naturally devoid of chymotryptic activity. This high quality trypsin is suitable for proteomics use.
Specific activity: >= 10,000 BAEE units per mg protein.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

supp_hazards

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 9

1 of 9

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service