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F1773

Sigma-Aldrich

Anti-CD4−FITC antibody, Mouse monoclonal

clone Q4120, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-CD4
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Q4120, monoclonal

form

buffered aqueous solution

mol wt

antigen 59 kDa

species reactivity

human

technique(s)

flow cytometry: 10 μL using 1 × 106 cells

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD4(920)

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This Item
SAB4700062F0772SAB5500064
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

Q4120, monoclonal

clone

MEM-241, monoclonal

clone

UCHT-4, monoclonal

clone

SP35, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human (tested)

Gene Information

human ... CD4(920)

Gene Information

human ... CD4(920)

Gene Information

human ... CD8A(925), CD8B(926)

Gene Information

human ... CD4(920)

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General description

Anti-CD4-FITC antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with cluster of differentiation 4 (CD4)-transfected mouse T cell hybridoma, 3DT, followed by CD4+ human T cell CEM cells (leukemic lymphoblasts).
CD4, a glycoprotein, is predominantly expressed on the cell membrane of mature, thymus-derived (T) lymphocyte and also to some extent in the monocyte/macrophage lineage. It has a transmembrane domain, cytoplasmic region and an extracellular region of four tandem domain similar like members of Ig superfamily.

Specificity

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Immunogen

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Application

FITC Conjugated Monoclonal Anti-Human CD4 has been used for:
  • identification, quantification and monitoring of helper/inducer T cells in peripheral blood, biological fluids, lymphoid organs, and other tissues
  • analysis of T cell mediated cytotoxicity
  • characterization of subtypes of T cell leukemias and lymphomas
  • studies of T cells in health and disease
  • flow cytometry

Biochem/physiol Actions

CD4+ T cells acts as a helper/inducer, in providing an activating signal to B cells. It induces T lymphocytes bearing the reciprocal CD8 marker to become cytotoxic/suppressor cells. It is also majorly associated with non-polymorphic region of MHC (major histocompatibility complex) antigen in interacting with targets bearing MHC class II molecules. Thus, it is called as an adhesion molecule. It also behaves as receptor for the GP 120 envelope glycoprotein of human immunodeficiency virus. The CD4 molecules is involved in the adhesion of T lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins.
Immunoregulatory T cell subset abnormalities in autoimmunity disorders, immunodeficiency diseases, graft versus host disease and following immunosuppressive therapy are often manifested as a change in CD4+/CD8+ ratio in peripheral blood T cells.

Target description

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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