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F5016

Sigma-Aldrich

Anti-Human IgG (Fc specific)−FITC antibody, Mouse monoclonal

clone HP-6017, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-Human IgG (Fc specific)
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

HP-6017, monoclonal

form

buffered aqueous solution

species reactivity

rabbit, sheep, horse (IgG), goat, human

storage condition

protect from light

technique(s)

dot immunobinding: 1:16
particle immunofluorescence: 1:16

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
F4516F0767B3773
conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

biotin conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

dot immunobinding: 1:16, particle immunofluorescence: 1:16

technique(s)

dot immunobinding: 1:32, particle immunofluorescence: 1:16

technique(s)

dot immunobinding: 1:64, particle immunofluorescence: 1:32

technique(s)

direct ELISA: 1:80,000

species reactivity

rabbit, sheep, horse (IgG), goat, human

species reactivity

-

species reactivity

-

species reactivity

rabbit, sheep, horse (IgG), goat, human

isotype

IgG2a

isotype

IgG1

isotype

IgG2a

isotype

IgG2a

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General description

Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. The light comprises of one variable N-terminal region and a constant C-terminal region. The heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

Specificity

The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.

Application

Monoclonal Anti-Human IgG (Fc specific)-FITC antibody produced in mouse has been used in:
  • Fluorescent Dot Immunobinding Assay (F-DIBA)
  • Particle Immunofluorescent Assay (F-IFMA)
  • flow cytometry

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), containing the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding.

Physical form

Solution in 0.01 M phosphate buffer pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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