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G211040

Sigma-Aldrich

DMT-2′O-TBDMS-rG(ib) Phosphoramidite

configured for MerMade

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Synonym(s):
N-(2-methyl-1-oxopropyl)-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-[(1,1-dimethylethyl)dimethylsilyl]-guanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
Empirical Formula (Hill Notation):
C50H68N7O9PSi
CAS Number:
Molecular Weight:
970.18
MDL number:
PubChem Substance ID:
NACRES:
NA.51

biological source

non-animal source (no BSE/TSE risk)

Quality Level

product line

Proligo Reagents

assay

≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)

form

powder

technique(s)

oligo synthesis: suitable

impurities

≤0.3% water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (reversed phase HPLC)
≤3% residual Solvent content

color

white to off-white

λ

conforms (UV/VIS Identity)

suitability

conforms to structure for H-NMR
conforms to structure for LC-MS

compatibility

configured for MerMade

nucleoside profile

base: guanosine
base protecting group: isobutyryl
2' protecting group: TBDMS
5' protecting group: DMT
deprotection: standard

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](O[Si](C)(C)C(C)(C)C)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(NC(=O)C(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6

InChI

1S/C50H68N7O9PSi/c1-32(2)45(58)54-48-53-44-41(46(59)55-48)52-31-56(44)47-43(66-68(12,13)49(7,8)9)42(65-67(63-29-17-28-51)57(33(3)4)34(5)6)40(64-47)30-62-50(35-18-15-14-16-19-35,36-20-24-38(60-10)25-21-36)37-22-26-39(61-11)27-23-37/h14-16,18-27,31-34,40,42-43,47H,17,29-30H2,1-13H3,(H2,53,54,55,58,59)/t40-,42-,43-,47-,67?/m1/s1

InChI key

PGJKESPHANLWME-FTZVBZPOSA-N

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This Item
G211000G211030A211040
Quality Level

300

Quality Level

300

Quality Level

-

Quality Level

300

assay

≥99% (31P-NMR), ≥99.0% (reversed phase HPLC)

assay

≥99% (31P-NMR), ≥99.0% (reversed phase HPLC)

assay

≥99% (31P-NMR), ≥99.0% (reversed phase HPLC)

assay

≥99% (31P-NMR), ≥99.0% (reversed phase HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

white to off-white

color

white to off-white

color

white to off-white

color

white to off-white

impurities

≤0.3% water content (Karl Fischer), ≤0.5% single Impurity (reversed phase HPLC), ≤0.5% P(III) Impurities 100-169ppm (31P-NMR)

impurities

≤0.3 wt. % water content (Karl Fischer), ≤0.5% P(III) Impurities 100-169ppm (31P-NMR), ≤0.5% single Impurity (reversed phase HPLC), ≤3 wt. % residual Solvent content

impurities

≤0.3% water content (Karl Fischer), ≤0.5% P(III) Impurities 100-169ppm (31P-NMR), ≤0.5% single Impurity (reversed phase HPLC), ≤3% residual Solvent content

impurities

≤0.3% water content (Karl Fischer), ≤0.5% P(III) Impurities 100-169ppm (31P-NMR), ≤0.5% single Impurity (reversed phase HPLC), ≤3% residual Solvent content

General description

RNA phosphoramidites, along with RNA CPG, and analogous to DNA synthesis support the synthesis of sequence-specific RNA oligonucleotides for RNA interference (RNAi) and can be used in target validation and other drug development techniques.Modern phosphoramidite-mediated synthesis has enabled routine high-yielding preparations of RNA oligonucleotides. High-quality RNA phosphoramidites are key to low failure rates, the high biological activity of synthesis products, and cost-effectiveness.Key Features
  • Industry-standard 2′O-TBDMS protective group
  • Consistent lot-to-lot purity and performance
  • Compatible with deprotection methods based on methylamine or AMA
  • Standard RNA phosphoramidites provide excellent coupling results whenused with ETT or BTT as an activator; best results are obtained withActivator 42®
  • Capping with standard acetic anhydride capping reagent rather than withFast Deprotection Cap A
  • Manufactured under a certified ISO 9001 quality systemDMT-2′O-TBDMS-rG(ib)
Phosphoramidite is configured for MerMade Synthesizers.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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