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G2580

Sigma-Aldrich

Sephadex® G-25

Fine

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CAS Number:
MDL number:
NACRES:
NA.56

technique(s)

LPLC: suitable

swelling

1 g swells to 4-6 mL

bead size

20-80 μm

application(s)

agriculture

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

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This Item
S5772G25150G25300
Sephadex® G-25 Fine

Sigma-Aldrich

G2580

Sephadex® G-25

Sephadex® G-25 BioReagent, for molecular biology, DNA grade, Superfine

Sigma-Aldrich

S5772

Sephadex® G-25

Sephadex® G-25 Medium

Sigma-Aldrich

G25150

Sephadex® G-25

Sephadex® G-25 Coarse

Sigma-Aldrich

G25300

Sephadex® G-25

application(s)

agriculture

application(s)

-

application(s)

-

application(s)

-

swelling

1 g swells to 4-6 mL

swelling

-

swelling

1 g swells to 4-6 mL

swelling

1 g swells to 4-6 mL gel

bead size

20-80 μm

bead size

-

bead size

50-150 μm

bead size

100-300 μm

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100, 200

General description

Sephadex® is a beaded gel filtration medium prepared by cross-linking dextran with epichlorohydrin under alkaline conditions. It is used in affinity chromatography, protein chromatography and gel filtration chromatography.
Fractionation Range (MW)
Globular Proteins: 1,000 - 5,000
Dextrans: 100 - 5,000

Application

Sephadex® G-25 has been used in size-exclusion chromatography to remove excess dye from labelled proteins.

Other Notes

G2580-100G′s updated product number is GE17-0032-01
G2580-500G′s updated product number is GE17-0032-02

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Celine Hoffmann et al.
Analytical biochemistry, 372(1), 62-71 (2007-09-11)
Fluorescein isothiocyanate (FITC) is a well-known probe for labeling biologically relevant proteins. However, the impact of the labeling procedure on protein structure and biological activities remains unclear. In this work, FITC-labeled human plasma fibronectin (Fn) was developed to gain insight
Jennifer Belyea et al.
Biochemistry, 45(48), 14275-14284 (2006-11-30)
The study of axial ligation by anionic ligands to ferric heme iron by resonance Raman spectroscopy provides a basis for comparison of the intrinsic electron donor ability of the proximal histidine in horse heart myoglobin (HHMb), dehaloperoxidase (DHP), and horseradish
Jana Schwarzer et al.
Electrophoresis, 29(20), 4203-4214 (2008-10-18)
Glycoproteins, such as monoclonal antibodies as well as recombinant and viral proteins produced in mammalian cell culture play an important role in manufacturing of many biopharmaceuticals. To ensure consisting quality of the corresponding products, glycosylation profiles have to be tightly
Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.
Mazouchi A, et al.
Biophysical Journal, 110(7), 1510-1522 (2016)
P M G Paiva et al.
Bioresource technology, 88(1), 75-79 (2003-02-08)
A highly purified trypsin inhibitor was obtained from Echinodorus paniculatus when an extract prepared from E. paniculatus seed flour (25 gl(-1), with 0.1 M ammonium acetate buffer, pH 8.3, under agitation for 6 min at 28 degrees C) was chromatographed

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