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G7781

Sigma-Aldrich

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-Glutathione-S-Transferase, Anti GST Antibody - Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit, Anti Gst Antibody, Anti-Gst Antibody, Gst Antibody, Anti-GST
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

packaging

antibody small pack of 25 μL

technique(s)

dot blot: suitable
indirect ELISA: 1:10,000
western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. The soluble GST is encoded by eight different genes in humans namely, α, μ, θ, π, ζ, σ, κ and ω on chromosome 6, 1, 22, 11, 14, 4, not known and 10 respectively.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Glutathione-S-transferase (GST) catalyzes the detoxication of electrophiles by glutathione conjugation. This enzyme provides protection against oxidative stress and toxic foreign chemicals. Polymorphism in GST gene is associated with the development of asthma and cutaneous basal cell carcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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