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G7914

Sigma-Aldrich

Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 46 kDa

species reactivity

human, rat

technique(s)

microarray: suitable
western blot: 1:2,000 using rat brain extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GSK3B(2932)
rat ... Gsk3b(84027)

General description

Anti-Glycogen Synthase Kinase-3β (GSK-3β) is developed in rabbit using a synthetic peptide (KGDRGQTNNAASASASNST) corresponding to the C-terminal region of human GSK-3β conjugated to KLH as immunogen. Glycogen Synthase Kinase-3β (GSK-3β) is present in cytoplasm, nucleus and mitochondria. GSK-3 exists as two closely related protein kinases, termed GSK-3α and GSK-3β. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids. It shows a wide range of tissue expression with the highest expression in brain.
Glycogen synthase kinase-3 β (GSK3β) is a 47kD protein that belongs to serine/ threonine protein kinase family and is expressed ubiquitously as a constitutively active kinase. It has a pivotal role in regulating the degradation of β-catenin by the tumor suppressor gene product APC. Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody can be used in microarray. Rabbit anti- GSK3β antibody reacts specifically with rat GSK3β but not with GSK3α.

Immunogen

Synthetic peptide corresponding to amino acids 403-420 of human glycogen synthase kinase 3 β (GSK3β), coupled to KLH. It is identical in rat and highly conserved in frog and zebrafish GSK3β.

Application

Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody produced in rabbit has been used in immunoblotting assays for analysis of protein expression.

Biochem/physiol Actions

Glycogen synthase kinase-3 (GSK-3) specifically phosphorylates glycogen synthase, a critical enzyme regulating glucose storage, and mediates insulin regulation of glycogen synthesis.(8) GSK-3β phosphorylates regulatory sites in the central region of APC. Binding of β-catenin to APC, marking β-catenin for destruction, is dependent on phosphorylation of APC by GSK-3β. Thus, GSK-3β may play a critical role in regulating β-catenin stability during development and tumor progression. It is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The Correlation of Glycogen Metabolism in Rabbit Myocardial Ischemia
Vale DF, et al.
Journal of Veterinary Science and Technology, 1(7) (2016)
B Rubinfeld et al.
Science (New York, N.Y.), 272(5264), 1023-1026 (1996-05-17)
The adenomatous polyposis coli gene (APC) is mutated in most colon cancers. The APC protein binds to the cellular adhesion molecule beta-catenin, which is a mammalian homolog of ARMADILLO, a component of the WINGLESS signaling pathway in Drosophila development. Here
Peutz-Jeghers LKB1 mutants fail to activate GSK-3beta, preventing it from inhibiting Wnt signaling
Lin-Marq N, et al.
Molecular Genetics and Genomics, 273(2), 184-196 (2005)
Site-specific phosphorylation protects glycogen synthase kinase-3beta from calpain-mediated truncation of its N and C termini
Ma S, et al.
The Journal of Biological Chemistry, 287(27), 22521-22532 (2012)
J Behrens et al.
Science (New York, N.Y.), 280(5363), 596-599 (1998-05-09)
Control of stability of beta-catenin is central in the wnt signaling pathway. Here, the protein conductin was found to form a complex with both beta-catenin and the tumor suppressor gene product adenomatous polyposis coli (APC). Conductin induced beta-catenin degradation, whereas

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