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Amersham Hybond® P Western blotting membranes, PVDF

pore size 0.45 μm, roll W × L 300 mm × 4 m, pkg of 1 ea

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shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pkg of 1 ea


Cytiva 10600023

roll W × L

300 mm × 4 m

pore size

0.45 μm pore size

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General description

PVDF membranes have higher mechanical strength than NC membranes and, thus, are more suitable for Western blotting applications where stripping and reprobing is needed. In addition, PVDF membranes exhibit higher protein binding capacity, however, with a tendency for a higher background, compared with NC membranes.

Features and Benefits

  • For use with standard colorimetric and chemiluminescent detection methods for proteins of > Mr 20 000
  • Offers high physical strength for easy signal detection
  • Provides high protein retention even after harsh washing steps
  • Captures high protein during transfer reduces the loss of sample
  • Available in precut sizes for laboratory uses

Legal Information

Amersham is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

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This page describes troubleshooting strategies in the detection of GST-tagged proteins.


This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

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