Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

GE25-0500-72

RNAspin Mini 250

greener alternative

Cytiva 25-0500-72

Synonym(s):

250 RNAspin Kit, Mini RNAspin 250, RNA Purification Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

manufacturer/tradename

Cytiva 25-0500-72

packaging

pkg of 250 preps

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

capacity

100 μg binding capacity

greener alternative category

General description

Amersham RNAspin Mini RNA Isolation Kit is designed for rapid extraction of high-quality RNA from a wide range of sample types.

Amersham RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast. The isolated RNA is of a high enough quality and quantity (up to 100 μg) for multiple downstream experiments and sensitive enzymatic applications like RT-PCR, primer extension, and RNase protection assays. The RNAspin Mini Kit has been used for the isolation of high-quality total RNA from several types of source material including hard-to-lyse bacterial strains, and applied in downstream experiments such as quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments. The kit is supplied with a full protocol booklet with a detachable, quick reference protocol card, and all the necessary components including prefilters and DNase I.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Used for the isolation of high-quality total RNA from a wide range of source material including hard-to-lyse bacterial strains. Purified RNA is suitable for use in sensitive downstream applications such as quantitative reverse transcriptase PCR (qRT-PCR), Northern blot and microarray experiments.

Features and Benefits

  • High-quality output RNA from diverse sample types.
  • Recovers high-quality total RNA due to the removal of genomic DNA through on-column DNase I treatment.
  • For maximum yield and purity, prefilters are included to reduce lysate viscosity.
  • Results can be obtained with even small amounts of precious sample.
  • Lysis buffer is less susceptible to foaming to ensure valuable RNA sample is not wasted.
  • Simple and convenient format that is suitable for all levels of expertise.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Amersham is a trademark of Cytiva

pictograms

Exclamation mark

signalword

Warning

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

This page shows possible causes and solutions for problems that may occur during RNA preparation.

This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.

Protocols

Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service