MilliporeSigma
All Photos(1)

Documents

GE25-8010-65

Sigma-Aldrich

CyDye DIGE Fluor Minimal Labeling Kit

Cytiva 25-8010-65, pack of 5 nmol

Sign Into View Organizational & Contract Pricing

NACRES:
NA.32

form

solid

shelf life

8‑12 mo. (for unreconstituted dye)
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

wetted part: no

packaging

pack of 5 nmol

manufacturer/tradename

Cytiva 25-8010-65

storage condition

protect from light

color

blue
red
yellow
Yellow, Red, Blue

solubility

cold water: soluble (Soluble in cold water and hot water)
soluble (in cold and hot water)

shipped in

dry ice

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
GE28-9345-30GE25-8010-85GE25-8010-83
CyDye™ DIGE Cy®5 Minimal pack of 5 nmol

GE25-8010-85

CyDye DIGE Cy®5 Minimal

CyDye™ DIGE Cy®3 Minimal pack of 5 nmol

GE25-8010-83

CyDye DIGE Cy®3 Minimal

form

solid

form

solid

form

-

form

-

solubility

cold water: soluble (Soluble in cold water and hot water), soluble (in cold and hot water)

solubility

cold water: soluble (Soluble in cold water and hot water)

solubility

-

solubility

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shelf life

8‑12 mo. (for unreconstituted dye), Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

-

shelf life

-

shelf life

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

CyDye DIGE Fluor minimal dyes and saturation dyes are size- and charge-matched fluorescent dyes specifically designed for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis using Ettan DIGE system.

CyDye DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection. In total, three minimal dyes and two saturation dyes are available.

CyDye DIGE fluors are exceptional dyes for multicolor analysis, offering bright and intense colors with narrow excitation and emission bands. The fluors are spectrally distinct, making them highly suitable for multicolor detection. CyDye DIGE fluors utilize these benefits but are also size- and charge-matched specifically for 2-D DIGE using Ettan DIGE system.
fluorescence color: blue, green, red

The dyes are dissolved in Dimethylformamide (DMF) and the minimal labeling reaction is quenched with L-Lysine

Features and Benefits

  • Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel.
  • Size- and charge-matched dyes enable co-migration of labeled samples within the gel.
  • Bright and highly sensitive dyes allow the use of the minimal labeling technique.
  • Minimal loss of signal during labeling, separation, and scanning.
  • No change in signal over wide pH range used during first-dimension (IEF) separation.
  • Discrete signal from each fluor with minimal cross-talk contributes to high accuracy.

Components

5 nmol of CyDye DIGE fluor minimal labeling kit (Cy2, Cy3 and Cy5)

Caution

avoid light, store in the dark

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

CyDye:
Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries.
CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva.
CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.
CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646.
Cy and CyDye, are - trademarks of Cytiva or one of its subsidiaries.
The purchase of CyDye products includes a limited license to use the CyDye products for internal research and development but not for any commercial purposes. A license to use the Cy and CyDye trademarks for commercial purposes is subject to a separate license agreement with Cytiva. Commercial use shall include:
1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
2. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.
3. Use of this material to perform services for a fee for third parties, including contract research and drug screening.
If you require a commercial license to use the Cy and CyDye trademarks please contact LSlicensing@cytiva.com.

If you require a commercial license to use this material and do not have one, return this material unopened to Cytiva Bio-Sciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden and any money paid for the material will be refunded.
CyDye is a trademark of Cytiva
Ettan is a trademark of Cytiva

pictograms

Exclamation mark

signalword

Danger

Storage Class

13 - Non Combustible Solids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Articles

This troubleshooting table lists problems that may be encountered in two-dimensional difference gel electrophoresis (2D DIGE) results, including possible causes and methods to prevent future problems.

Protocols

This page describes two-dimensional separation of protein samples with Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service