Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE28-9574-99

TALON® Superflow

Cytiva 28-9574-99, pack of 10 mL

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 28-9574-99

matrix

6% cross-linked agarose

particle size

60-160 μm

cleaning in place

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

capacity

≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

storage temp.

2-8°C

Related Categories

General description

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media.

Features and Benefits

  • Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 3

1 of 3

Articles

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

See All

Protocols

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service