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GE29-0181-83

HiPrep™ SP High Performance16/10

Cytiva 29-0181-83, pack of 1 column

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 1 column

manufacturer/tradename

Cytiva 29-0181-83

parameter

1-5 ml/min flow rate
44 psi

bed size

16 mm × 100 mm

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

Related Categories

General description

HiPrep SP HP 16/10 is a strong cation exchanger designed for preparative ion exchange chromatography separations. This 20 mL column is prepacked with SP Sepharose High Performance chromatography resin. The resin is based on rigid, highly cross-linked beaded agarose with a mean bead diameter of 34 µm for high resolution separations. High mechanical strength of the matrix allows for high flow rates, together with outstanding resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.

Features and Benefits

  • For high-performance preparative scale separations where high efficiency and high resolution are desired.
  • Highly cross-linked agarose provides excellent chemical and physical stability.
  • Slim, modern design allows for easy handling and connection.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva

related product

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service