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H0164

Sigma-Aldrich

Anti-Histone H3 antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti H3 Antibody, Histone H3 Antibody, Histone H3 Antibody - Anti-Histone H3 antibody produced in rabbit
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 17 kDa

species reactivity

chicken, mouse, plant, Drosophila, rat, human, Xenopus

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cells
western blot: 1:2,500-1:5,000 using whole cell extract of the rat pheochromocytoma PC12 cell line
western blot: 1:5,000-1:10,000 using whole cell extract of the A431 human epidermoid carcinoma cell line or a whole cell extract of the mouse fibroblast NIH3T3 cell line

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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SAB4500352SAB4500353SAB4503284
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

mol wt

antigen 17 kDa

mol wt

antigen 15 kDa

mol wt

antigen 15 kDa

mol wt

antigen 15 kDa

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

Histone proteins are known to stabilize and regulate the nuclear DNA. Core histones, H2A, H2B, H3 and H4 aid the packaging of eukaryotic nuclear DNA into repeating nucleosome units while the linker histone, H1, binds to DNA between the nucleosomes. A diverse and elaborate array of post-translational modifications, including acetylation, phosphorylation and methylation, ubiquitination and ADP ribosylation occur in histones, particularly on the N-terminal tail domains of histones, particularly of H3 and H4.These modifications dictate chromatin states and the gene expression.
Phosphorylation of H3, mainly on Ser10 and Ser28, is localized to a small fraction of highly acetylated H3 and occurs primarily in response to mitogenic and stress stimuli. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Histone methylation, like acetylation, is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis ad heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9, and Lys27 are the preferred sites of methylation. Histone lysine residues can be mono-, di-, and tri-methylated at different heterochromatic subdomains, adding further complexity to the regulation of chromatin structure.

Specificity

Anti-Histone H3 specifically recognizes histone H3.

Immunogen

synthetic peptide corresponding to amino acids 125-136 located at the C-terminus of human histone H3, conjugated to KLH. This sequence is identical in many species including rat, mouse, chicken, Xenopus, Drosophila, and plant histone H3.

Application

Anti-Histone H3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Evidence that core histone H3 is targeted to the mitochondria in Brassica oleracea mitochondria in Brassica oleracea
Katherine B Zanin M et al
Cell Biology International, 34, 997-1003 (2012)
MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction.
Thomson S et al
Seminars in Cell Biology, 10, 205-214 (1999)
Jiejing Li et al.
Molecular medicine reports, 10(6), 2805-2810 (2014-09-23)
Nicotinic acid (NA), a member of the vitamin B family, is well known for its functions in the treatment and prevention of atherosclerosis due to decreasing plasma levels of low-density lipoprotein cholesterol. In recent years, the major side effect of
M J Barratt et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(11), 4781-4785 (1994-05-24)
Diverse agents, including growth factors and phorbol esters, induce rapid transcriptional activation of a subset of immediate-early (IE) genes that include the protooncogenes c-fos and c-jun. Among the earliest nuclear signaling events concomitant with IE gene activation is the phosphorylation
Histone H3 is absent from organelle nucleoids in BY-2 cultured tobacco
cells
Takusagawa M
Cell Biology International, 37, 748-754 (2013)

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