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H8283

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Hemocyanin from Megathura crenulata (keyhole limpet)

PBS solution

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Synonym(s):
KLH, Keyhole limpet hemocyanin
CAS Number:
MDL number:

biological source

Megathura crenulata

Quality Level

form

PBS solution

concentration

3.0-7.0 mg/mL protein (A280)

copper content

0.10-0.30% (based on protein)

technique(s)

ELISA: suitable

storage temp.

2-8°C

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This Item
H7017374825374807
form

PBS solution

form

powder

form

liquid

form

liquid

concentration

3.0-7.0 mg/mL protein (A280)

concentration

-

concentration

-

concentration

-

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

100

General description

Gastropod hemocyanins are massive glycoproteins (4 to 8 MDa) designed by an intricate arrangement of 10 subunits that are self-assembled into hollow cylinders 35 nm in diameter.
Hemocyanin is a high molecular weight copper containing glycoprotein. It reversibly binds oxygen and forms the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, also called the giant keyhole limpet. It is native to the southern California coast and Mexico.

Application

Keyhole limpet hemocyanin (KLH) is a strong and well known immune-stimulant in both experimental animals and humans. It has been used as a hapten carrier protein to enhance antigen-specific T cell priming and is known to stimulate a CD4+ T-cell response.
Hemocyanin was used to study if antipeptide antibodies specifically recognize the l1 protein of the human papilloma virus of type 31. It was also used to study the acid-induced unfolding of didecameric keyhole limpet hemocyanin.

Physical form

Solution in PBS, pH 7.2, containing 1mM CaCl2 and 0.5 mM MgCl2 with 15 mM sodium azide as preservative

Preparation Note

Chromatographically purified.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

Slide 1 of 3

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S M Andreev et al.
Bioorganicheskaia khimiia, 38(6), 667-675 (2013-04-04)
Antibodies that specifically recognize the capsid protein (L1) of human papillomavirus (HPV) are an important tool necessary for designing vaccines against HPV infection. In this work, we have predicted and synthesized peptide fragments mimicking B cell epitopes of L1 HPV
Ankita Varshney et al.
Amino acids, 39(3), 899-910 (2010-03-10)
Keyhole limpet hemocyanin (KLH) is widely used as an immune stimulant and hapten carrier derived from a marine mollusc Megathura crenulata. To provide details of the stability and equilibrium of KLH, different intermediate species were investigated with a series of
Juan Cordero-Solorzano et al.
Genetics, selection, evolution : GSE, 54(1), 68-68 (2022-10-24)
A sufficient IgG content in the colostrum is essential for the newborn calf, as it provides passive immunity which substantially affects the probability of survival during rearing. Failure of passive transfer (FPT) occurs when a calf does not absorb enough
Hendrikus J Wijnen et al.
Frontiers in veterinary science, 8, 784869-784869 (2022-01-01)
Resilient animals can cope with environmental disturbances in life with minimal loss of function. Resilience can be enhanced by optimizing early-life conditions. In poultry, eggshell temperature (EST) during incubation and early feeding are two early-life conditions that are found to
Traumatic aneurysm of the internal carotid artery with fracture dislocation at C 5: case report.
H Wada et al.
Neurosurgical review, 12 Suppl 1, 600-601 (1989-01-01)

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