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HPA019163

Sigma-Aldrich

Anti-CCL2 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

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Synonym(s):
Anti-C-C motif chemokine 2, Anti-HC11, Anti-MCAF, Anti-MCP-1, Anti-Monocyte chemoattractant protein 1, Anti-Monocyte chemotactic and activating factor, Anti-Monocyte chemotactic protein 1, Anti-Monocyte secretory protein JE, Anti-Small-inducible cytokine A2
Human Protein Atlas Number:

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

enhanced validation

orthogonal RNAseq
Learn more about Antibody Enhanced Validation

technique(s)

immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

PDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CCL2(6347)

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This Item
SAB1411231SAB5700155HPA024578
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

-

product line

-

product line

Prestige Antibodies® Powered by Atlas Antibodies

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

CCL2 is a dimer and structurally comprises a triple-stranded antiparallel β-sheet, greek key motif and an irregular β- strand at N-terminus.
The gene CCL2 (C-C motif chemokine 2) is mapped to human chromosome 17q11.2-q12. It belongs to chemoattractant cytokines family. CCL2 is also referred to as MCP1 (monocyte chemoattractant protein 1).

Immunogen

C-C motif chemokine 2 Precursor recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.
Anti-CCL2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

Proteins belonging to chemoattractant cytokines family mainly induce the migration and accumulation of different white blood cells. CCL2 (C-C motif chemokine 2) signaling via CCR2 (C-C chemokine receptor type 2) is crucial for recruitment of macrophages to tissue lesions or inflammation sites. CCL2 is up-regulated in luminal B breast cancer cells. It controls cell growth, inhibits necrosis and autophagy. CCL2 participates in activation of spinal microglia through PI3K/AKT (phosphatidylinositol 3-kinase/protein Kinase B) signaling pathway in bone cancer pain (BCP). Isoglutaminyl cyclase mediated CCL2 maturation in brain causes neuro-inflammation in Alzheimer′s disease. Expression levels of CCL2 in synovial fluid are linked with pain and physical disability in osteoarthritis patients.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST86662

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Di Jin et al.
Brain research, 1599, 158-167 (2015-01-04)
Accumulating evidence suggests that chemokine monocyte chemoattractant protein-1 (MCP-1) is significantly involved in the activation of spinal microglia associated with pathological pain, at the same time that the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/Akt) pathway localized in spinal microglia is involved
Tatsuya Yoshida et al.
Clinical and experimental gastroenterology, 13, 427-438 (2020-10-17)
Colorectal cancer (CRC) often recurs after curative resection. Identification of major risk factors for CRC recurrence is important for effective prevention and treatment. In this study, we examined the potential relationship between CRC and TLR3 as this remains unclear. Correlations
Kouji Izumi et al.
EMBO molecular medicine, 5(9), 1383-1401 (2013-08-29)
Increased CCL2 expression in prostate cancer (PCa) cells enhanced metastasis via macrophage recruitment. However, its linkage to androgen receptor (AR)-mediated PCa progression remains unclear. Here, we identified a previously unrecognized regulation: targeting AR with siRNA in PCa cells increased macrophage
Robert D Loberg et al.
Cancer research, 67(19), 9417-9424 (2007-10-03)
The identification of novel tumor-interactive chemokines and the associated insights into the molecular and cellular basis of tumor-microenvironment interactions have continued to stimulate the development of targeted cancer therapeutics. Recently, we have identified monocyte chemoattractant protein 1 (MCP-1; CCL2) as
The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions
Lubkowski J, et al.
Nature Structural Biology, 4(1), 64-69 (1997)

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