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I1408

Sigma-Aldrich

IMAC-Select Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Synonym(s):

Affinity Gel

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Quality Level

200

form

(1:1 suspension in a 30% ethanol solution)

shelf life

1 yr

technique(s)

affinity chromatography: suitable

impurities

≤0.08 Metal Contamination (μmol/ml packed gel)

matrix

6% beaded agarose

capacity

10-30 μmol/mL binding capacity (Cu+2)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Application

An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.

Physical form

1:1 suspension in a 30% ethanol solution

Preparation Note

The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Other Notes

Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

120.2 °F - closed cup

flash_point_c

49 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Kris Gevaert et al.
Methods in molecular biology (Clifton, N.J.), 527, 219-227 (2009-02-26)
We present a gel-free proteomics procedure for the specific isolation of phosphorylated peptides from whole proteome digests. Central is the use of diagonal, reverse-phase chromatography which consists of two consecutive reverse-phase peptide separations with a modification step in between. The
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
The Journal of biological chemistry, jbc-M111 (2012)
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
Test, jbc-M111 (2012)
The hantavirus glycoprotein G1 tail contains dual CCHC-type classical zinc fingers
Estrada DF, et al.
The Journal of Biological Chemistry, 284(13), 8654-8660 (2009)
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
The Journal of Biological Chemistry, jbc-M111 (2012)
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