Documents
I5513
Imidazole
for molecular biology, ≥99% (titration)
Recommended Products
grade
for molecular biology
Quality Level
vapor pressure
<1 mmHg ( 20 °C)
Assay
≥99% (titration)
form
crystalline powder
pH
6.2-7.8 (25 °C)
pKa (25 °C)
6.95
bp
256 °C (lit.)
mp
88-91 °C (lit.)
solubility
water: 500 mg/mL, clear, colorless to very faintly yellow
application(s)
diagnostic assay manufacturing
foreign activity
DNase, RNase and protease, none detected
storage temp.
room temp
SMILES string
c1c[nH]cn1
InChI
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
InChI key
RAXXELZNTBOGNW-UHFFFAOYSA-N
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Related Categories
1 of 4
This Item | I202 | I2399 | 4015 |
|---|---|---|---|
| assay ≥99% (titration) | assay 99% | assay ≥99% (titration) | assay ≥99% (1H-NMR) |
| form crystalline powder | form crystalline | form - | form solid |
| pH 6.2-7.8 (25 °C) | pH 9-11 at 100 g/L (23 °C) | pH 9.5-11.0 (25 °C, 5% in H2O) | pH - |
| mp 88-91 °C (lit.) | mp 88-91 °C (lit.) | mp 88-91 °C (lit.) | mp - |
| solubility water: 500 mg/mL, clear, colorless to very faintly yellow | solubility - | solubility - | solubility water: 100 mg/mL |
General description
Furthermore, imidazole is indispensable for enhancing protein purity. It is widely used in preparing buffer solutions optimized for downstream purification columns. Imidazole solutions are also effective elution and washing agents throughout protein purification processes. Additionally, imidazole can be used for assays of horseradish peroxidase (HRP) and reverse staining of SDS-PAGE gels for protein detection. Imidazole is a versatile and valuable reagent for a wide range of biological applications. It is an essential component of many buffers and is also used in protein purification and detection protocols.
Application
- in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
- in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
- as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
- as a binding buffer, wash buffer, and elution buffer in a study involving protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
Biochem/physiol Actions
Features and Benefits
- Suitable for Molecular Biology and Biochemical Research
- Can be used as a Buffer component, for Protein separation
- Free from DNase, NICKase, RNase, and Protease
- Effective Buffering from pH 6.2 to 7.8 (25 °C) with a pKa of 6.9 (25 °C)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Storage Class Code
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flash Point(F)
293.0 °F - closed cup
Flash Point(C)
145 °C - closed cup
Personal Protective Equipment
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