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L3410

Sigma-Aldrich

Anti-Leptin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Leptin Antibody, Leptin Antibody - Anti-Leptin antibody produced in rabbit, Anti-OB
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

indirect ELISA: 1:1,000 using human or mouse recombinant leptin
microarray: suitable
western blot: 1:5,000 using human recombinant leptin

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LEP(3952)
mouse ... Lep(16846)

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This Item
L3160L1418F3648
biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

form

buffered aqueous solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

mouse, human

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

human

technique(s)

indirect ELISA: 1:1,000 using human or mouse recombinant leptin, western blot: 1:5,000 using human recombinant leptin, microarray: suitable

technique(s)

indirect ELISA: suitable, radioimmunoassay: suitable, western blot: 1:1,000 using recombinant human leptin

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa, rat NRK, and mouse NIH3T3 cells

technique(s)

indirect ELISA: 1:10,000 using human fibronectin, indirect immunofluorescence: 1:400 using human foreskin cultured fibroblasts, microarray: suitable, western blot: 1:1,000 using human plasma fibronectin

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General description

The gene for leptin (LEP) is mapped to the human chromosome 7q32.1. The encoded protein is a non-glycosylated polypeptide, primarily produced and secreted by the mature adipocytes. Leptin is expressed in white adipose tissue, fetal tissues, skeletal muscle, chondrocytes, gastric chief cells, the placenta, ovary, and many immune cells. It is absent or expressed at extremely low levels in other adult tissues. Human leptin is 85% identical to mouse leptin and 84% identical to rat leptin, while mouse and rat exhibit 96% identity in their leptin molecules.

Specificity

Anti-Leptin reacts specifically with leptin.

Immunogen

synthetic peptide corresponds to the amino terminal (amino acid residues 22-40) of human/mouse leptin.

Application

Anti-Leptin antibody produced in rabbit has been used in:
  • western blotting
  • immunohistochemistry
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Leptin is a circulating cytokine that induces pleiotropic effects on nutrient intake, thermogenesis, metabolism, reproduction and hematopoiesis. Leptin is hypothesized to be a “satiety factor” because the absence of this factor is associated with hyperphagia and obesity in ob/ob mice (homozygous for the obese spontaneous mutation). It appears that leptin is involved in appetite regulation since leptin injection into ob/ob mice reduces their food intake and ultimately their body weight. However, studies have demonstrated that its effects must be more complicated than simple appetite suppression.
Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism and gastric mobility. The pathways that mediate the effect of leptin are Janus kinase (JAK)/signal transducer and activator of transcription (STAT), phosphoinositide 3-kinase (PI3K) and c-Jun N-terminal kinase (JNK).

Physical form

Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

The antibody is affinity isolated on peptide-agarose.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Leptin regulates food intake and energy expenditure (EE) and is produced in adipocytes, the pituitary, and several other tissues. Animals that are leptin or leptin receptor deficient have major metabolic complications, including obesity. This study tests the hypothesis that the
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Obesity is associated with low-grade chronic inflammation attributed to dysregulated production, release of cytokines and adipokines and to dysregulated glucose-insulin homeostasis and dyslipidemia. Nutritional interventions such as dieting are often accompanied by repeated bouts of weight loss and regain, a
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The aim of this study was to evaluate the expression of leptin and its receptor in histological sections of prostate tumors, and their association with prognostic factors. A total of 532 surgical specimens from prostate cancer were studied. After histopathological
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Leptin is an adipokine that is overexpressed in obese and overweight people. Interestingly, women with breast cancer present high levels of leptin and of its receptor ObR. Leptin plays an important role in breast cancer progression due to the biological

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