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Magnesium chloride hexahydrate

BioReagent, suitable for cell culture, suitable for insect cell culture

MgCl2 hexahydrate, MgCl2.6H2O
Linear Formula:
MgCl2 · 6H2O
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

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Quality Level


cell culture | insect: suitable
cell culture | mammalian: suitable


H2O: soluble 500 mg/mL, clear, colorless

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cell culture | insect: suitable







divalent cation, cell attachment, cell adherence, adenovirus infections





Magnesium chloride hexahydrate (MgCl2·6H2O) has been used:
  • to prepare electrochemical substrate solution for surface treatment and immunoassay preparation of monoclonal anti-creatine kinase (CK-MB) antibody
  • as a component of casamino acids medium (Hv-Ca) to culture Haloferax volcanii strains
  • to prepare hydrated-salt poly(vinylidenefluoride)-hexafluoropropylene P(VDF-HFP)/MgCl2·6H2O nanocomposite film to study its phase transformation, surface morphology and dielectric property

Biochem/physiol Actions

Magnesium chloride is commonly used as a reagent in chemistry and molecular biology as a source of magnesium ions. Magnesium exhibits several biological roles in enzymology, cell membrane and wall structural integrity, muscle cell physiology, and nucleic acid structure. It functions as a co-factor in various enzymes, like deoxyribonuclease (DNAse), the restriction enzymes EcoRI and EcoRV, and Ribonuclease H. Magnesium stabilizes polymeric nucleic acids including ribozymes and transfer RNA.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Sodium chloride BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%



Sodium chloride

Calcium chloride anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%



Calcium chloride

Roshali T de Silva et al.
Microbiology (Reading, England), 167(2) (2021-01-19)
Some microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt to changed environmental conditions. The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped ('plate') cells in culture, but may also be present as rods
Vladimir Riazanski et al.
iScience, 23(11), 101759-101759 (2020-12-01)
The relative contribution of the two phagosomal catabolic processes, oxidative and metabolic, was assessed in the killing of Pseudomonas aeruginosa in phagosomes of alveolar macrophages (AMs) from wild-type (p47-phox +/+ ) or NOX-defective (p47-phox -/- ) mice. Free radical release
Goli Ardestani et al.
Cell calcium, 87, 102181-102181 (2020-02-26)
Prior to maturation, mouse oocytes are arrested at the germinal vesicle (GV) stage during which they experience constitutive calcium (Ca2+) influx and spontaneous Ca2+ oscillations. The oscillations cease during maturation but Ca2+ influx continues, as the oocytes' internal stores attain
Alissa C Rothchild et al.
Bio-protocol, 10(10) (2020-10-01)
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events
Huiwen Hao et al.
EMBO reports, 21(3), e48385-e48385 (2020-01-28)
Microtubules derived from the Golgi (Golgi MTs) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT-dependent vesicular trafficking. Here, using super-resolution

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