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M2393

Sigma-Aldrich

Magnesium chloride hexahydrate

BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym(s):

MgCl2 hexahydrate, MgCl2.6H2O

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About This Item

Linear Formula:
MgCl2 · 6H2O
CAS Number:
Molecular Weight:
203.30
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.71

grade:
reagent
solubility:
H2O: soluble 500 mg/mL, clear, colorless

grade

reagent

product line

BioReagent

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

solubility

H2O: soluble 500 mg/mL, clear, colorless

SMILES string

O.O.O.O.O.O.Cl[Mg]Cl

InChI

1S/2ClH.Mg.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2

InChI key

DHRRIBDTHFBPNG-UHFFFAOYSA-L

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Application

Magnesium chloride hexahydrate (MgCl2·6H2O) has been used:
  • to prepare electrochemical substrate solution for surface treatment and immunoassay preparation of monoclonal anti-creatine kinase (CK-MB) antibody
  • as a component of casamino acids medium (Hv-Ca) to culture Haloferax volcanii strains
  • to prepare hydrated-salt poly(vinylidenefluoride)-hexafluoropropylene P(VDF-HFP)/MgCl2·6H2O nanocomposite film to study its phase transformation, surface morphology and dielectric property

Biochem/physiol Actions

Magnesium chloride is commonly used as a reagent in chemistry and molecular biology as a source of magnesium ions. Magnesium exhibits several biological roles in enzymology, cell membrane and wall structural integrity, muscle cell physiology, and nucleic acid structure. It functions as a co-factor in various enzymes, like deoxyribonuclease (DNAse), the restriction enzymes EcoRI and EcoRV, and Ribonuclease H. Magnesium stabilizes polymeric nucleic acids including ribozymes and transfer RNA.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Goli Ardestani et al.
Cell calcium, 87, 102181-102181 (2020-02-26)
Prior to maturation, mouse oocytes are arrested at the germinal vesicle (GV) stage during which they experience constitutive calcium (Ca2+) influx and spontaneous Ca2+ oscillations. The oscillations cease during maturation but Ca2+ influx continues, as the oocytes' internal stores attain
Huiwen Hao et al.
EMBO reports, 21(3), e48385-e48385 (2020-01-28)
Microtubules derived from the Golgi (Golgi MTs) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT-dependent vesicular trafficking. Here, using super-resolution
Alissa C Rothchild et al.
Bio-protocol, 10(10) (2020-10-01)
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events
Yuxiang Jiang et al.
Bio-protocol, 7(5), e2146-e2146 (2017-03-05)
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing the dynamics of actin filaments at single-filament resolution in vitro. Thanks to the development of various fluorescent probes, we can easily monitor all kinds of events associated with
Beeke Wienert et al.
Nature protocols, 15(5), 1775-1799 (2020-04-22)
DISCOVER-seq (discovery of in situ Cas off-targets and verification by sequencing) is a broadly applicable approach for unbiased CRISPR-Cas off-target identification in cells and tissues. It leverages the recruitment of DNA repair factors to double-strand breaks (DSBs) after genome editing

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