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M5293

Sigma-Aldrich

Monoclonal Anti-Mucin Gastric antibody produced in mouse

clone 45M1, ascites fluid

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MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

45M1, monoclonal

contains

15 mM sodium azide

species reactivity

chicken, monkey, rat, rabbit, pig, hedgehog, mouse, human, feline

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using sections of human stomach
western blot: suitable using non-reducing conditions

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MUC5AC(4586)
mouse ... Muc5ac(17833)
rat ... Muc5ac(682837)

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biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

chicken, monkey, rat, rabbit, pig, hedgehog, mouse, human, feline

species reactivity

guinea pig, human, pig, canine, rabbit, chicken

species reactivity

human

species reactivity

human

clone

45M1, monoclonal

clone

hSM-V, monoclonal

clone

2H7, monoclonal

clone

5B12, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

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General description

Monoclonal Anti-Human Gastric Mucin (mouse IgG1 isotype) is derived from the 445M1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with mucin isolated from human ovarian cyst fluid. Mucin is a high M.W. (1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in Müller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).
Mucin has a netlike, branched structure and is a secreted protein. It belongs to the category of gel-forming mucins. The gene encoding this protein is upregulated by interleukin-1β (IL-1β) and is localized on chromosome 11p15.5.

Specificity

The antibody recognizes the mucin epitope g located in the peptide core of gastric mucin (>1,000 kDa). This epitope is completely destroyed by thiol reduction (using mercaptoethanol) and partially lost following trypsin proteolysis, but is stable upon periodate oxidation. The antibody stains the surface gastric epithelium of normal human gastrointestinal tract and reacts with fetal, pre-cancerous and cancerous colonic mucosa, but not with normal colon.

Immunogen

mucin from human ovarian cyst fluid.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-Human Gastric Mucin may be used for the localization of gastric mucin using various immunochemical assays such as immunoblot, immunofluorescence, immunohistochemistry, immunostaining and immunoradiofixation.

Biochem/physiol Actions

Mucin is composed of a peptide core containing heavily glycosylated regions and nonglycosylated regions. The heavily glycosylated components of mucin consist of a mosaic of epitopes, some of which are associated with saccharide moieties and are related to blood-group antigens, and others which are M1 antigens associated with the peptidic core. M1 antigens are common to human ovarian mucinous cyst fluids and to gastric mucosa. M1 is distributed in various human tumors such as the colon, stomach, pancreas, ovary, Barrets′ esophagus, endocervix, and endometrium carcinomas.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Genome reference and sequence variation in the large repetitive central exon of human MUC5AC.
Guo X
American Journal of Respiratory Cell and Molecular Biology, 50(1), 223-232 (2014)
Abnormal expression of M1/MUC5AC mucin in distal colon of patients with diverticulitis, ulcerative colitis and cancer.
Forgue-Lafitte ME, et al.
International Journal of Cancer. Journal International Du Cancer, 121(7), 1543-1549 (2007)
Blocking peripheral serotonin synthesis by telotristat etiprate (LX1032/LX1606) reduces severity of both chemical-and infection-induced intestinal inflammation.
Kim JJ, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(6), G455-G465 (2015)
Gastrointestinal cell lines form polarized epithelia with an adherent mucus layer when cultured in semi-wet interfaces with mechanical stimulation.
Navabi N, et al.
PLoS ONE, 8(7), e68761-e68761 (2013)
Optimized Protocol for Regeneration of the Conjunctival Epithelium Using the Cell Suspension Technique.
Bertolin M, et al.
Cornea, 38(4), 469-479 (2019)

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