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MAK053

Sigma-Aldrich

Alcohol Dehydrogenase Activity Assay Kit

sufficient for 100 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

storage temp.

−20°C

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WH0000130M1SAB1410225SAB1405430
detection method

colorimetric

detection method

-

detection method

-

detection method

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Gene Information

human ... ADH1A(124), ADH1B(125), ADH1C(126), ADH4(127), ADH5(128), ADH6(130), ADH7(131)
mouse ... ADH1C(11522), ADH4(26876), ADH5(11532), ADH6A(69117), ADH7(11529)
rat ... ADH1C(24172), ADH4(29646), ADH5(100145871), ADH6(310903), ADH6A(295498), ADH7(171178)

Gene Information

human ... ADH6(130)

Gene Information

human ... ADH6(130)

Gene Information

human ... ADH4(127)

relevant disease(s)

gastrointestinal diseases

relevant disease(s)

-

relevant disease(s)

-

relevant disease(s)

-

General description

The new Alcohol Dehydrogenase (ADH) Assay Kit, MAK498, is now available! Alcohol dehydrogenases (ADH) are a family of enzymes that catalyzes the conversion of alcohols to aldehydes, with the concomitant reduction of NAD+ to NADH. In humans, there are nine isozymes of ADH, with the majority of ADH activity occurring in the liver. ADH family members are the primary enzymes involved in alcohol detoxification. Genetic variations in ADH enzymes result in differences in ADH activity and tolerances for alcohol, and may regulate susceptibility to alcoholism.

Application

Alcohol Dehydrogenase Activity Assay Kit has been used to determine the activity of alcohol dehydrogenase.

Suitability

Suitable for the detection of alcohol dehydrogenase in a variety of samples including tissue, cells and serum.

Principle

The Alcohol Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring ADH activity in a variety of samples. ADH activity is determined using ethanol as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of ADH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at 37 °C.

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Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12.0 °C - closed cup


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Alcohol increases liver progenitor populations and induces disease phenotypes in human IPSC-derived mature stage hepatic cells
Tian L, et al.
International Journal of Biological Sciences, 12(9), 1052-1052 (2016)
Christopher M Ranger et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(17), 4447-4452 (2018-04-11)
Animal-microbe mutualisms are typically maintained by vertical symbiont transmission or partner choice. A third mechanism, screening of high-quality symbionts, has been predicted in theory, but empirical examples are rare. Here we demonstrate that ambrosia beetles rely on ethanol within host
Rachel E N Reyes et al.
Brain research, 1747, 147067-147067 (2020-08-23)
Research focusing on the gut-brain axis is growing, but the interplay of ethanol (alcohol molecule), the gut microbiome, the brain and behavior is poorly understood. In the current study, we remodeled the gut microbiota by providing adult male C57BL/6J mice
Kobi Simpson-Lavy et al.
Scientific reports, 9(1), 6491-6491 (2019-04-26)
Cells adapt their gene expression and their metabolism in response to a changing environment. Glucose represses expression of genes involved in the catabolism of other carbon sources in a process known as (carbon) catabolite repression. However, the relationships between "poor"
Lipeng Tian et al.
International journal of biological sciences, 12(9), 1052-1062 (2016-08-30)
Alcohol consumption has long been a global problem affecting human health, and has been found to influence both fetal and adult liver functions. However, how alcohol affects human liver development and liver progenitor cells remains largely unknown. Here, we used

Protocols

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

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