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MAK072

Sigma-Aldrich

Pyruvate Kinase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

hematological disorder; cancer

storage temp.

−20°C

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MAK332MAK102P0294
vibrant-m

MAK072

Pyruvate Kinase Activity Assay Kit

vibrant-m

MAK332

Pyruvate Assay Kit

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

-

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

-

relevant disease(s)

hematological disorder; cancer

relevant disease(s)

gastrointestinal diseases; cancer

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

-

General description

Pyruvate kinase (PK) is an enzyme that catalyzes the final step of glycolysis, the transfer of a phosphate group from phospho(enol)pyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, due to defects in pyruvate kinase expression or activity, is the second most common cause of hemolytic anemia.

The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.

Application

Pyruvate Kinase Activity Assay Kit has been used to determine the activity of pyruvate kinase in samples.

Suitability

Suitable for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc

Principle

The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.

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Health hazard

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Articles

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

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