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MAK246

Sigma-Aldrich

Neutrophil Elastase Activity Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

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NACRES:
NA.25

detection method

fluorometric

relevant disease(s)

hematological disorder; pulmonary disorders

storage temp.

−20°C

General description

Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). Mutation in the gene ELA2 (neutrophil elastase) results in neutropenia, a hereditary disorder characterized by reduced neutrophil count in an individual.

Application


  • Relationship between airway inflammation and airflow limitation in elderly asthmatics.: This study investigates the connection between airway inflammation and airflow limitation in elderly asthmatic patients. It highlights the role of neutrophil elastase in airway inflammation, which was measured using the Neutrophil Elastase Activity Assay Kit (Fluorometric). The findings suggest that targeting neutrophil elastase activity could help in managing airflow limitation in elderly asthmatics, offering potential therapeutic applications for the assay kit (Soma et al., 2020).

Suitability

Suitable to determine the activity of purified Neutrophil Elastase, detect the activity of Neutrophil Elastase in plasma and/or blood and screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase.

Principle

The Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence. This assay kit is simple, rapid, and can detect as low as 1 ng of neutrophil elastase in a variety of samples.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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